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. 2008 May 14;105(20):7252–7256. doi: 10.1073/pnas.0800272105

Fig. 3.

Fig. 3.

Alk7 is expressed in adipose tissue and can mediate GDF3 signaling in a Cripto dependent manner. (A) RT-PCR analysis of Gdf3, Alk7, Alk4, ActRII-A, ActRII-B, and Cripto mRNA expression. Gapdh mRNA expression is shown in the bottom-most panel as a loading control. “no input” indicates that no mRNA was used for RT-PCR. (B) Luciferase reporter assay in R4-2 cells transfected with a CAGA-luc reporter and plasmids encoding mature GDF3, ALK7, and Cripto as indicated. Results are expressed in relative luciferase units normalized to control (mean ± SD of triplicate observations). (C) Luciferase reporter assay in HepG2 cells transfected with a BRE-luc reporter and plasmids encoding mature GDF3, Cripto, and chimeric type I receptor ALK7/3 as indicated. BMP4 protein (50 ng/ml) was used as a positive control for reporter activation.