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Effects of morphine and rhGH treatment on the density of neurons in primary cultures of fetal mouse hippocampal neurons. Cells were treated with different concentrations of morphine (A–C and E) alone or in the presence of rhGH (F) for 7 days. Shown are representative light microscopic images. (A) Morphine, 0.1 μM. (B) Morphine, 1 μM. (C) Morphine, 20 μM. (D) Untreated cells. (E) Morphine, 15 μM. (F) Morphine (15 μM) together with rhGH (1 μM). (Magnification: ×20.)
