Table 1.
Resting dimension of isolated ductus arteriosus from WT and eNOS−/− fetal mice in the absence and presence of indomethacin pretreatment
| Condition | Internal diameter |
Vessel lengtha |
Wall thickness | |
|---|---|---|---|---|
| Short side | Long side | |||
| WT, control (n=13) | 130±1 | 581±20 | 594±20 | 21±0.5 |
| WT, acute indo (n=5) | 120±2* | 503±33 | 512±31 | 19±0.9 |
| WT, chronic indo (n=12) | 128±1 | 525±16 | 540±16 | 20±0.6 |
| eNOS−/−, control (n=7) | 136±1 | 567±13 | 577±12 | 19±0.8 |
| eNOS−/−, chronic indo (n=8) | 132±2 | 537±20 | 546±21 | 20±0.7 |
Abbreviations: eNOS, endothelial NOS; indo, indomethacin; WT, wild type.
Values (μm) are mean±s.e.mean and apply to preparations used for in vitro studies. Pregnant mothers were treated with vehicle alone (control) or indomethacin in single or repeated doses (respectively, acute and chronic treatments; for details, see Methods).
a
Vessel length of the ductus is uneven because of its insertion into aorta at an angle (short side taken for calculations, see In vitro studies).
*P<0.05 vs WT control and eNOS−/− chronic indo; P<0.001 vs eNOS−/− control (ANOVA with Bonferroni post hoc test).