Effects of 5 h treatment with galegine (a, upper panel) and insulin (b, lower panel) on 2-deoxy-D-glucose uptake by L6 myotubes in the absence or presence of 1 μM wortmannin, 100 μM LY294002 or 10 μM cytochalasin B. Results are expressed as a percentage of basal glucose uptake and values shown are mean±s.e.m. of observations from two individual experiments with three replicates per experiment. *P<0.05, **P<0.01, ***P<0.001 vs galegine alone.