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HGF induces GSK-3β phosphorylation in the obstructed kidney in vivo. A: Kidney homogenates obtained from various groups as indicated were immunoblotted with specific antibodies against phosphorylated GSK-3β and GAPDH, respectively. Numbers (1 to 3) denote each individual animal. B: Relative phosphorylated GSK-3β abundance (fold induction over sham controls) was calculated and presented as mean ± SEM of three animals per group (n = 3). *P < 0.05 versus sham or pcDNA3 controls.
