Figure 6.

HGF does not affect IL-1β-mediated phosphorylation of IκBα and p65 NF-κB, as well as the degradation and abundance of IκBα and IκBβ. HKC-8 cells were treated with IL-1β (5 ng/ml) alone or IL-1β plus HGF (40 ng/ml). At different time points as indicated, cells were harvested and subjected to immunoblotting with antibodies against p-IκBα, IκBα, IκBβ (Α), p-p65 NF-κB, and p65 NF-κB (B), respectively. C: Long-term incubation with HGF also did not affect the phosphorylated status of IκBα and p65 NF-κB in HKC-8 cells.