Figure 2.

Corneal neovascularization stimulated by VEGF-C and VEGF. Pellets of sucrose aluminum sulfate and hydron polymer containing 160 ng of VEGF-C (A) and VEGF (B) were implanted into corneal micropockets of C57BL6/J mice as described in Materials and Methods. Corneas were photographed with a slit-lamp stereomicroscope on day 5 after growth factor implantation. Dashed lines encircle the area of the implanted pellets. Photographs represent ×20 amplification of the mouse eye. Quantitation of corneal neovascularization. (C) Maximal vessel length. (D) Clock hours of circumferential neovascularization. (E) Area of neovascularization. Graphs represent mean values (±SEM) of 10 eyes (five mice) in each group. The P values were calculated by two-tailed Student t test analysis using the statistical instat 1.1 and Microsoft excel 5 programs.