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Cell proliferation. The proliferative effects of VEGF-C (■) and VEGF (○) were assayed on PAE/VEGFR-1 (A), PAE/VEGFR-2 (B), and PAE/VEGFR-3 (C) cells. VEGF-C and VEGF at various concentrations in a triplicate of each sample were incubated with cells in the presence of 1% FCS. After 72 h incubation, cell numbers were counted by a Coulter counter, and values represent the mean (±SEM) of a triplicate of each sample. PBS was used as the negative control.
