Table 1.

The mismatches at the 3' end and the second mismatches at the third or other position from the 3' end in an allele-specific primer

IUB/IUPAC Code of a SNP	Alleles of a SNP	Mismatch at the 3' end (forward/reverse)	Mismatch strength at the 3' end	Second mismatch at the third or other position from the 3' end
R	G/A	G/T	Weak	G/A, T/C
		A/C	Weak	A/G, C/T
Y	T/C	G/T	Weak	G/A, T/C
		A/C	Weak	A/G, C/T
S	G/C	G/G	Medium	C/C, A/A, G/G, T/T
		C/C	Medium	C/C, A/A, G/G, T/T
W	A/T	A/A	Medium	C/C, A/A, G/G, T/T
		T/T	Medium	C/C, A/A, G/G, T/T
K	G/T	G/A	Strong	G/T, A/C
		T/C	Strong	T/G, C/A
M	A/C	G/A	Strong	G/T, A/C
		T/C	Strong	T/G, C/A