Table 3.
Composition and geometric means (GM) of mouse IgG anti-Shewanella MR-4 CPS induced by BSA conjugates of CPSTSB and CPSCDM
| Conjugate | Protein/saccharide ratio, wt/wt | GM of IgG after 3rd injection, EU |
|
|---|---|---|---|
| Coating antigen CPSTSB | Coating antigen CPSCDM | ||
| BSA/CPSTSB | 2:1 | 54 | 43 |
| BSA/CPSCDM | 0.7:1 | 31 | 32 |
Mice (10 per group) were injected s.c. with 2.5 μg saccharide as a conjugate/mouse 3 times (2 wk apart) and killed 1 wk after the last injection. Antibody levels were calculated relative to a hyperimmune anti-Shewanella spp. MR-4 mouse serum that was assigned a value of 100 EU.