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. 2008 Jun 23;105(25):8736–8741. doi: 10.1073/pnas.0803281105

Fig. 3.

Fig. 3.

Essential gene analysis. The neutral base pair model was used to estimate the number of ORFs missed in the library due to chance alone. The difference between the number of ORFs that were not hit in the library (gray bars) and the number of ORFs likely not hit by chance (striped bars) represent ORFs that are likely essential in V. cholerae (white bars). Of the 48 ORFs larger than 3 kb that are not shown on the graph, 3 were not hit in the library (the RNA polymerase subunits rpoB and rpoC and the DNA polymerase dnaE). These ORFs are very likely to be essential. ORFs are grouped by size in 250-bp increments.