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. 2008 Jun 18;105(25):8597–8600. doi: 10.1073/pnas.0801643105

Fig. 4.

Fig. 4.

EPR analysis of the reaction of the high-valent MauG intermediate with its natural substrate. (a) High-valent MauG intermediate spectrum generated from reaction of di-ferric MauG with a stoichiometric amount of H2O2. (b) Spectrum of a further reacted with a stoichiometric amount of the biosynthetic precursor of MADH for 15 s before freezing in liquid nitrogen. (Inset) Spectrum of the g = 2 region. (c) Parallel sample of b further reacted with 2 mM hydroxyurea for 2 min. EPR parameters were temperature 10 K, microwave power 0.5 mW and modulation amplitude 5 G.