Figure 1.

Lsi6 Gene Expression.

(A) Transcripts of Lsi1, Lsi2, and Lsi6 were detected by RT-PCR in wild-type rice cv Nipponbare. Total RNA was isolated from the root (R), leaf sheath (LS), and leaf blade (LB) of 4-week-old seedlings grown hydroponically. Actin was used as an internal standard.

(B) Effect of Si supply on Lsi6 expression. Four-week-old seedlings (cv Nipponbare) grown hydroponically were treated with or without 0.5 mM Si for 6 d, and then relative expression levels of Lsi6 in the root, leaf sheath, and leaf blade were compared by quantitative RT-PCR. Means ± sd of biological replicates (n = 3) are shown.

(C) Lsi6 and Lsi1 expression in the root. The primary roots of hydroponically grown 5-d-old rice seedlings (cv Nipponbare) were used. The region 0 to 40 mm from the root tips was separated into four segments as indicated in the figure, and the transcript levels were quantified by RT-PCR. Means ± sd of biological replicates (n = 3) are shown.