XCT Is a Widely Expressed, Nuclear-Localized Protein.
(A) to (D) XCT-YFP-HA expressed under the control of the native XCT promoter is found in the nuclei of cells throughout the plant, including trichome (A), hypocotyl (B), root (C), and root meristem (D). Right panels show YFP signal alone (imaged by fluorescence microscopy), and an overlay of the YFP signal with bright field image is presented in the left panels. Bars = 25 μm.
(E) Six-day-old xct-2 seedlings display a delayed greening of cotyledons and young tissues, which is rescued by transformation with genomic XCT under the control of the native promoter. Bar = 1 mm.