Figure 7.

MTA Interacts with FIP37.

(A) MTA interacts with FIP37 (b) but does not interact with the empty prey vector, pDEST22 (a). FIP37 does not transactivate GUS expression in the presence of the empty bait vector, pDEST32 (c). ProQuest (Invitrogen) controls for assessing the relative interaction strengths are indicated 1 to 5.

(B) Proteins of wild-type and transgenic Arabidopsis plants expressing MTA-c-Myc were extracted under nondenaturing conditions and immunoprecipitated using anti-c-Myc antibody. After SDS-PAGE separation, the anti-FIP37 antibody detected a protein of 48 kD (bottom arrow) in the precipitated extracts of plants expressing the MTA-c-Myc fusion but not in those of the wild-type control.

(C) Transient expression of fluorescent protein fusions in onion epidermal peels. FIP37-CFP (left) and MTA-YFP (middle) colocalized to the nucleus. YFP and CFP images were superimposed on the bright-field image to show the position of the nucleus (right). Bars = 10 μm.