Table 2.
Monosaccharide Composition and Linkage Analysis of RGI/XGA Isolated from Adult Leaves from the Wild Type (qrt) and xgd1-1
| qrt | xgd1-1 | |
|---|---|---|
| Monosaccharide Composition (mol %) | ||
| Fuc | 2.7 | 3.2 |
| Rha | 19.2 | 20.9 |
| Ara | 26.1 | 27.5 |
| Gal | 9.2 | 15.0 |
| Glc | Trace | Trace |
| Xyl | 3.8 | 1.2 |
| GalA | 37.9 | 30.8 |
| GlcA | 1.0 | 1.4 |
| Linkage Analysis (mol %) | ||
| 2-Rha | 15.6 | 12.9 |
| 2,4-Rha | 8.4 | 10.5 |
| t-Xyl | 4.6 | 1.1 |
| t-Gal | 7.4 | 9.0 |
| 4-Gal | 8.9 | 12.8 |
| 3-Gal | 3.4 | 3.1 |
| 6-Gal | 3.4 | 2.4 |
| 3,6-Gal | 5.4 | 4.3 |
| 3,4-Gal | 1.5 | 1.2 |
| 4,6-Gal | 0.7 | 1.0 |
| t-Araf | 4.9 | 5.9 |
| 5-Araf | 17.7 | 18.6 |
| 2,5-Araf | 3.0 | 3.4 |
| 2,3,5-Araf | 2.7 | 4.1 |
| t-Fuc | 0.7 | 0.8 |
| 4-GalA | 33.4 | 28.4 |
| 3,4-GalA | 4.5 | 2.4 |
A reduction in t-Xyl in xgd1-1 could be observed in both the monosaccharide composition and in the linkage analysis, and a reduction in 3,4-GalA was also observed. The neutral sugars and uronic acids were determined in separate analyses, and the combined mol % of 4-GalA and 3,4-GalA was calculated according to the monosaccharide composition (top). No other sugars or sugar derivates than those mentioned in the table could be detected. Similar results were obtained in two independent experiments.