Figure 5. Peptide exchange results for human SH3 domains.

Upper heat maps show the topological distortion (left) and the energy variations (right) of all artificial (i.e. non-native) interaction pairs constructed between human SH3 domains and their ligand proteins, with respect to the native topologies and the average native binding energy, respectively. SH3-binding peptides 1–9 correspond to class I and 10–16 to Class II. The lower figure shows the energy contribution of motif and context, with respect to the native binding energies, for each individual residue in the native (n) and artificial (a) interactions, determined by in silico alanine scanning. Detailed information is provided for the consensus motifs.