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. 1998 Nov 24;95(24):14400–14405. doi: 10.1073/pnas.95.24.14400

Figure 6.

Figure 6

Expression of Bcl-2, Bax, and Bak in normal breast epithelial and breast carcinoma cells infected with Ad. mda-7 S or Ad.Vec. (A) Immunoblot analyses of BAX protein in normal mammary epithelial and cancer cells. Equal amounts of whole cell protein (verified by Coomassie blue staining) from 2- and 4-day cell cultures infected with 100 pfu/cell of Ad.Vec or Ad.mda-7 S were resolved by SDS/PAGE (4–20%), were immunoblotted, and were probed with Anti-BAX mAb. Note that both intact BAX and processed BAX proteins are visible in the breast cancer cell lines 2 days after Ad.mda-7 infection. Low levels of BAX in 4-day MCF-7 is caused by proteolytic degradation because the cells in this study were 70% apoptotic by 4 days after infection with Ad.mda-7 S. (B) Tabular compilation of protein levels of the Bcl-2 gene family members, BCL-2 and BAK, 4 days after infection with 100 pfu/cell of Ad.mda-7 S or Ad.Vec in normal breast epithelial cells and breast carcinoma cell lines.