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. Author manuscript; available in PMC: 2008 Nov 14.
Published in final edited form as: FEBS Lett. 2008 Apr 11;582(11):1575–1580. doi: 10.1016/j.febslet.2008.03.056

Figure 1. Der1p and Dfm1p self-associate, but do not interact with each other.

Figure 1

(A) Yeast cells expressing an HA-tagged version of Der1p (Der1-3HA) with or without a SA-tagged version of Der1p (Der1-SA) were lysed. Samples were analyzed directly (input; 5% of total material) or after immunoprecipitation using coupled S-peptide binding protein (SBP) (IP; 95% of total material). All samples were separated by SDS-PAGE and analyzed by immunoblotting (IB) with the indicated antibodies. The arrowhead indicates Der1-3HA co-immunoprecipitated by Der1-SA. (B) As in (A), but using cells expressing tagged versions of Dfm1p (Dfm1-CA and Dfm1-3HA, respectively). The IP was performed using bead-coupled calmodulin (CAL). The arrowhead indicates Dfm1-3HA co-immunoprecipitated by Dfm1-CA. The asterisk indicates Dfm1-CA detected by IgG of the secondary antibody. (C) As in (B) but using cells expressing tagged versions of Dfm1p and Der1p (Dfm1-CA and Der1-3HA, respectively).