Figure 2. Interaction partners of Der1p and Dfm1p.

Wild-type yeast cells (con) or cells expressing either Der1-SA or Dfm1-CA were lysed and membrane fractions were solubilized with 1% digitonin. The extract was incubated with IgG-coupled magnetic beads and bound material was analyzed by SDS-PAGE and Coomassie staining. The numbered stained bands were cut out and their identity determined by mass spectrometry. Brackets indicate where closely spaced bands were cut out and analyzed together.