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. Author manuscript; available in PMC: 2008 Nov 14.
Published in final edited form as: FEBS Lett. 2008 Apr 11;582(11):1575–1580. doi: 10.1016/j.febslet.2008.03.056

Figure 3. Der1p and Dfm1p bind a different set of Cdc48p cofactors.

Figure 3

(A) Yeast cell lysates from cells expressing a tagged version of Der1p (Der1-3HA) and a tagged version of either Ubx1p (Ubx1-SA), Ufd1p (Ufd1-SA) or Ubx7p (Ubx7-SA) were analyzed either directly (input; 5% of total material) or by immunoprecipitation (IP; 95% of total material) using coupled S-peptide binding protein (SBP). The samples were separated by SDS-PAGE and analyzed by immunoblotting (IB) with the indicated antibodies. The arrowhead in the upper panel indicates Der1-3HA co-immunoprecipitated by Ufd1-SA. The square bracket in the lower panel indicates the position of the SA-tagged cofactors. (B) As in (A), except that a tagged version of Dfm1p (Dfm1-3HA) was expressed together with the SA-tagged cofactors. The immunoprecipitation was performed using IgG bound to magnetic beads (IgG). The arrowhead in the upper panel indicates Dfm1-3HA co-immunoprecipitated by Ubx1-SA, Ufd1-SA, or Ubx7-SA. The square bracket in the lower panel indicates the position of the SA-tagged cofactors. The asterisks indicate coeluted IgG and a crossreacting protein.