Table.
Summary of interacting proteins found when immunoprecipitation was performed using tagged Der1p (Der1-SA) or tagged Dfm1p (Dfm1-CA). The bound proteins were identified using mass spectrometry. We analyzed either the total protein eluate after precipitation with trichloroacetic acid (TCA), or individual protein bands after their separation in a SDS gel and staining with Coomassie blue (Ind). Numbers indicate the amount of peptides identified by mass spectromety. For multiple experiments, numbers are separated by a dash. The numbers in brackets correspond to the numbers assigned to the bands in Figure 2.
| Interacting protein | Tagged protein (Bait) | |||
|---|---|---|---|---|
| Der1-SA [5] | Dfm1-CA [6] | |||
| TCA | Ind | TCA | Ind | |
| Cdc48p | 12 | 23 [1] | 46/68 | 55 [1] |
| Usa1p | 10 | 23 [2] | 2/6 | 21 [2] |
| Hrd3p | 11 | 23 [2] | 2/9 | 22 [2] |
| Kar2 | 0 | 0 | 0/8 | 31 [3] |
| Ubx2p | 0 | 8 [3] | 0/0 | 7 [4] |
| Yos9p | 5 | 6 [3] | 2/1 | 10 [4] |
| Hrd1p | 1 | 9 [3] | 3/2 | 7 [5] |
| Npl4p | 0 | 3 [3] | 0/0 | 8 [5] |
| Ubx1p | 0 | 0 | 4/4 | 2 [6] |
| Ubx7p | 0 | 0 | 4/8 | 5 [6] |
| Ufd1p | 0 | 1 [5] | 0/0 | 0 |