Figure 5. Disruption of Cajal bodies by CMV UL3 and UL30.

(A) UL3- and UL30- expressing U2OS cells and untransfected control cells were stained with FLAG antibody to detect the viral protein (green) and coilin antibody to detect Cajal bodies (red) and counterstained with DAPI (blue). Scale bar  =  10 µm. (B) Histogram showing the average number of Cajal bodies per cell in cells prepared as in (A). Mean values±SE from 5 separate experiments are shown. (C) Data from samples in (B) were plotted to show the percentage of cells with a given number of Cajal bodies per cell. Mean values±SE from 3 separate experiments are shown.