Table 2. Characteristics of the 14 microsatellite markers used for population analysis of Mediterranean Leishmania infantum .
Nr. | Marker | Population | n | Repeat array | Fragment size array [bp] | A | H e | H o | F IS |
1 | Lm2TG | MON-1 | 113 | TG12–27 | 116–146 | 9 | 0.758 | 0.071 | 0.906 |
non-MON-1 | 26 | TG 12–29 | 116–150 | 11 | 0.848 | 0.154 | 0.821 | ||
2 | TubCA | MON-1 | 113 | CA9–13 | 80–88 | 2 | 0.018 | 0 | 1.000 |
non-MON-1 | 26 | CA 9–16 | 80–94 | 5 | 0.523 | 0.038 | 0.928 | ||
3 | Lm4TA | MON-1 | 113 | TA 4–16 | 63–87 | 9 | 0.768 | 0.036 | 0.954 |
non-MON-1 | 26 | TA 9–14 | 73–83 | 4 | 0.533 | 0.038 | 0.929 | ||
4 | Li 41–56 | MON-1 | 113 | CA 9–12 | 88–94 | 3 | 0.155 | 0.009 | 0.943 |
non-MON-1 | 26 | CA 9–13 | 88–96 | 4 | 0.338 | 0.077 | 0.776 | ||
5 | Li 46–67 | MON-1 | 113 | CA 6–9 | 74–80 | 2 | 0.018 | 0.018 | −0.004 |
non-MON-1 | 26 | CA 6–9 | 74–80 | 4 | 0.709 | 0.231 | 0.679 | ||
6 | Li 22–35 | MON-1 | 113 | CA 10–25 | 88–118 | 10 | 0.690 | 0.080 | 0.885 |
non-MON-1 | 26 | CA 6–28 | 80–124 | 13 | 0.876 | 0.192 | 0.784 | ||
7 | Li 23–41 | MON-1 | 113 | GT 7–17 | 67–87 | 5 | 0.294 | 0.027 | 0.910 |
non-MON-1 | 26 | GT 15–23 | 83–99 | 8 | 0.786 | 0.269 | 0.662 | ||
8 | Li 45–24 | MON-1 | 113 | CA 13–22 | 101–119 | 6 | 0.505 | 0.044 | 0.913 |
non-MON-1 | 26 | CA 7–20 | 89–115 | 6 | 0.643 | 0.077 | 0.882 | ||
9 | Li 71–33 | MON-1 | 113 | TG 11–12 | 105–107 | 2 | 0.052 | 0 | 1.000 |
non-MON-1 | 26 | TG 11–27 | 105–137 | 7 | 0.534 | 0.231 | 0.573 | ||
10 | Li 71-5/2 | MON-1 | 99 | CA 8–16 | 108–124 | 4 | 0.302 | 0.020 | 0.933 |
non-MON-1 | 26 | CA 7–9 | 106–110 | 3 | 0.274 | 0 | 1.000 | ||
11 | Li 71-7 | MON-1 | 113 | CA 12–14 | 98–102 | 3 | 0.148 | 0.035 | 0.761 |
non-MON-1 | 26 | CA 8–13 | 90–100 | 5 | 0.518 | 0.038 | 0.927 | ||
12 | CS20 | MON-1 | 113 | TG 17–19 | 81–85 | 3 | 0.331 | 0 | 1.000 |
non-MON-1 | 26 | TG 18–22 | 83–91 | 5 | 0.750 | 0.154 | 0.798 | ||
13 | LIST7031 | MON-1 | 113 | CA 10–12 | 109–113 | 3 | 0.353 | 0.045 | 0.874 |
non-MON-1 | 26 | CA 10–12 | 109–113 | 3 | 0.528 | 0.077 | 0.857 | ||
14 | LIST7039 | MON-1 | 113 | CA 14–17 | 205–211 | 3 | 0.165 | 0.018 | 0.892 |
non-MON-1 | 26 | CA 15–20 | 207–217 | 6 | 0.729 | 0.077 | 0.896 | ||
overall | MON-1 | 112 | 4.6 | 0.325 | 0.029 | 0.912 | |||
non-MON-1 | 26 | 5.6 | 0.614 | 0.118 | 0.810 |
A, number of alleles; n, sample size; H o, observed heterozygosity; H e, expected heterozygosity; F IS, inbreeding coefficient. Two of the four heterozygous strains with mixed MON-1/non-MON-1 alleles (PT2(I) and INF-32) have been removed from the data set, in case of ES9(III) and ES10(III) only the non-MON-1 genotype has been considered, as these strains have been identified as MON-24 by MLEE.