Table 3.
Tumorigenic activity of mutationally activated Met in NIH 3T3 cells under the control of the metallothionein promoter
| MET construct* | Tumorigenicity†
|
|
|---|---|---|
| No. mice with tumors/no. mice injected | Mean tumor size in mm2 | |
| Wild type | 0/5 | 0 |
| M1268T | 5/5 | 234 |
| Y1248H | 5/5 | 238 |
*
The wild-type construct encodes murine Met; M1268T and Y1248H encode mutationally activated murine Met. Each Met molecule is in vector 2999, which utilizes the metallothionein promoter.
†
NIH 3T3 cells were cotransfected with the indicated construct plus pSV2 Neo, selected as pools of G418-resistant cells, and injected s.c. into nude mice at 5 × 105 cells per animal. Tumors were measured after 19 days. Mice injected with cells expressing wild-type Met developed tumors at 4–5 weeks.