Table 1.
Effects of cannabinoids on spontaneous PC excitatory spike properties
| PC firing rate (%) | CTL | BMI | +5 μM WIN55 | +5 μM Δ9-THCV | +10 μM Δ9-THCV | +20 μM Δ9-THCV | +40 μM Δ9-THCV |
|---|---|---|---|---|---|---|---|
| CTL (n=28 (6)) | 100 | — | 216±21* | 49±17* | 27±15* | 15±8* | 14±8* |
| 10 μM BMI (n=38 (6)) | 100 | 346±46* | 536±83* | 224±86 | 125±7† | 162±10† | 135±10† |
| 30 μM BMI (n=35 (6)) | 100 | 564±62* | 587±17a | 598±18a | 581±17a | 620±45a | 626±23a |
Abbreviations: BMI=bicuculline methiodide; CTL=control; PC=Purkinje cell; Δ9-THCV=Δ9-tetrahydrocannabivarin; WIN55=212-2, (R)-(+)-[2,3-dihydro-5-methyl-3-(4-morpholinylmethyl)pyrrolo-[1,2,3-de]-1,4-benzoxazin-6-yl]-1-naphthalenylmethanone mesylate.
PC firing rate (expressed as a percentage of control firing rate) calculated using Neuroexplorer3 (Nex Technologies), MATLAB (Mathworks Inc.) plus MEA Tools (Egert et al., 2002b) as described in Methods. Note: Δ9-THCV results were obtained in the continued presence of 5 μM WIN55.
a
NS=no significant difference vs BMI control, Mann–Whitney U-test.[3]*P<0.05 vs control, †P<0.05 vs BMI control.