Table 2.

Oligonucleotide primer and probe sets used for the amplification of gene expression in T84 cells

Gene	Sequence
ABCB1 (MDR1)
Forward	5′-GTCCCAGGAGCCCATCCT-3′
Reverse	5′-CCCGGCTGTTGTCTCCATA-3′
Probe	5′-TTGACTGCAGCATTGCTGAGAACATTG C-3′
NR1I2 (PXR)
Forward	5′-CGAGCTCCGCAGCATCA-3′
Reverse	5′-TGTATGTCCTGGATGCGCA-3′
Probe	5′-CGGTGCCATCCCTTGACTCAACCT-3′
NR1I3 (CAR)
Forward	5′-CCAAATCCAGCACATCCAGG-3′
Reverse	5′-GCCTCAGCTGCAGATCTCCT-3′
Probe	5′-GTCTGCCATGATGCCGCTGCT-3′

Abbreviations: ABCB1, ATP-binding cassette B1; CAR, constitutive androstane receptor; MDR1, multi-drug resistance 1; PXR, pregnane X receptor.