Figure 3.

Histopathology and TdT-mediated dUTP nick end labelling (TUNEL) detection of apoptosis in the heart of experimental mice (original magnification, × 200). Heart tissues from normal, As₂O₃-treated and As₂O₃+resveratrol (Rev)-treated mice were sectioned at 5 μm. These slides were processed for HE (hematoxylin and eosin) staining (a) and TUNEL assay (b). Quantitative analysis of apoptotic nuclei is shown in (c). The number of apoptotic nuclei was significantly increased in As₂O₃-treated hearts compared with the control group (^*P<0.01, n=8 hearts, five sections counted per heart). Fewer apoptotic nuclei were found in the As₂O₃+Rev groups compared with the As₂O₃-treated group (^*P<0.01).