Figure 1.

Identification of CNI-1493-unresponsive cells. RAW 264.7 cell cultures were exposed to CNI-1493 1 hr before LPS stimulation, and TNF levels in the supernatant were determined by ELISA 4 hr after LPS treatment. All data are expressed as % of control (+ LPS alone). For RAW 264.7 cells precultured under normal conditions, CNI-1493 promoted a dose-dependent suppression of TNF release (□), with an estimated IC₅₀ of 1.5 μM. When RAW 264.7 cells were precultured under serum-deprived conditions, they became unresponsive to CNI-1493 (○).