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. 1998 Nov 24;95(24):14429–14434. doi: 10.1073/pnas.95.24.14429

Figure 2.

Figure 2

Determination by preparative SDS/PAGE gel elution of the protein responsible for CNI-1493-enhancing activity. (A) SDS/PAGE. The Mono-Q fractions containing the major CNI-1493-enhancing activity were pooled, fractioned on a 4–20% gradient polyacrylamide gel, and stained with silver. Two major bands with molecular masses of 65–67 and 55–56 kDa along with several minor bands in the range of 15–30 kDa were detected. (B) SDS/PAGE gel elution. Duplicate lanes of the preparative SDS/PAGE gel were sliced (at 2 mm in width). Proteins in each slice were eluted into 1 × PBS and added concurrently with CNI-1493 in the LPS challenge assay. CNI-1493-enhancing activity was eluted from only one slice, containing the prominent 65-to 67-kDa protein band.