Figure 3.

Effect of fetuin on binding and uptake of CNI-1493 to macrophages. RAW 264.7 cells were precultured under serum-deprived conditions until confluency, resuspended into fresh RPMI medium 1640/10% FBS, and plated on 96-well tissue culture plate at 5 × 10⁵ cells/well per 0.2 ml. After incubation at 37°C for 2 hr to allow adherence, radiolabeled ¹⁴C-CNI-1493 was added to the cultures either alone or in the presence of increasing concentrations of fetuin (0–50 μg/ml) and incubated for 30 min at 4°C or 37°C. After three extensive washings, the cell-associated radioactivity (cpm/well per 5 × 10⁶ cells) was measured and expressed as mean ± SEM (n = 4).