Figure 5.

Effect of human fetuin (α₂-HS-glycoprotein) on CNI-1493-mediated suppression of TNF production. (A) Effect of human fetuin (α₂-HS-GP) on CNI-1493-mediated suppression of HuPBMCs. Human α₂-HS-glycoprotein was added concurrently with 0.5 μM CNI-1493. Four hours after LPS stimulation, TNF levels in the supernatants were measured and expressed as % of control (+ LPS alone). (B) Effect of α₂-HS-glycoprotein-specific antibodies on suppression of HuPBMCs by CNI-1493. Polyclonal antiserum against human α₂-HS-glycoprotein was added (5.0 or 50 μl/ml) with CNI-1493 (1.0 μM) 1 hr before LPS stimulation. Note that human fetuin-specific antiserum abrogated the suppression of HuPBMCs by CNI-1493.