Figure 2.

BAX induction after irradiation of GBM- and MB-derived cell lines. (A) Representative Northern blot analysis of the expression BAX. Total RNA prepared from U563, U343, U87, D283, and D341 at 0 (1), 6 (2), and 24 (3) hr after treatment with 5 Gy of ionizing radiation was examined by Northern blot analysis for expression of BAX and GAPDH mRNA. (B) The densitometric intensity of bands corresponding to BAX mRNA was divided by the intensity of the GAPDH bands for each time point and expressed as fold induction on the bar graph. The expression of BAX mRNA at the 0-hr time point was arbitrarily set at one for each cell line, and the degree of induction was determined by comparing the level of expression at 6 and 24 hr after irradiation. Each bar represents the mean ± SD of the results of three independent experiments.