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. 1981 Jun;41(6):1482–1483. doi: 10.1128/aem.41.6.1482-1483.1981

Possible Relationship Between Yeast Deoxyribonucleases and Deoxyribonucleic Acid Yield

Hamza El-Nakhal 1, H J Phaff 2
PMCID: PMC243944  PMID: 16345800

Abstract

The deoxyribonucleic acid (DNA) yield and deoxyribonuclease (DNase) activity of several yeasts were correlated. Debaryomyces castellii and Debaryomyces franciscae were found to contain active DNases which carry out DNA hydrolysis, whereas the amounts of DNA as determined by extraction with Sarkosyl buffer (pH 7.8) were found to be small. On the other hand, Candida parapsilosis, Saccharomyces carmosousae, and Lodderomyces elongisporus produced no detectable DNases active at pH 7.8, and their DNA yield was correspondingly high. L. elongisporus was found to possess DNase only at pH 4.0.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Fedorcsák I., Ehrenberg L. Effects of diethyl pyrocarbonate and methyl methanesulfonate on nucleic acids and nucleases. Acta Chem Scand. 1966;20(1):107–112. doi: 10.3891/acta.chem.scand.20-0107. [DOI] [PubMed] [Google Scholar]
  2. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  3. Price C. W., Fuson G. B., Phaff H. J. Genome comparison in yeast systematics: delimitation of species within the genera Schwanniomyces, Saccharomyces, Debaryomyces, and Pichia. Microbiol Rev. 1978 Mar;42(1):161–193. doi: 10.1128/mr.42.1.161-193.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Rosén C. G., Fedorcsák I. Studies on the action of diethyl pyrocarbonate on proteins. Biochim Biophys Acta. 1966 Dec 28;130(2):401–405. doi: 10.1016/0304-4165(66)90236-4. [DOI] [PubMed] [Google Scholar]

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