Figure 1.

In vitro transcription and translation of Pax1 and derived mutants. The in vitro transcription/translation products of wt-Pax1 (wt), sb-derived-Pax1 (sb), and un-Pax1 (un) were detected on Western blot by a specific anti-Pax1 antibody. As a positive (pc) and negative control (nc) total protein extract from the developing vertebral column of 13.5-day-old wt and a homozygous undulated shorttail (Un^S) embryos were used, respectively. In Un^S mutant mice, the Pax1 gene has been deleted completely. The antibody detects a predicted band of ≈40 kDa, which is not present in the negative control. No Pax1 was detected in the transcription/translation lysate (not shown).