Figure 2.

Electrophoretic mobility shift assays with Pax1 and derived mutants. In vitro transcription/translation products of wt-Pax1, sb-derived Pax1, and un-Pax1 were assayed for binding activity on the PRS4 probe derived from the e5 site in the Drosophila even-skipped promoter. The lanes Ly, un, wt, sb, SC, and 0, correspond to incubation of the probe with reticulocyte lysate, un-Pax1, wt-Pax1, sb-Pax1, and wt-Pax1 + unlabeled PRS4 probe in a 500-fold excess, and H₂O, respectively. The Pax1 bands are indicated with arrows. Nonspecific bands are indicated with asterisks.