Uptake of 5-HT in peripheral arteries. (a) Confocal image of freshly dissociated rat aortic smooth muscle cells double stained with pan-cadherin antibody (Sigma) and SERT antibody (C-20, Santa Cruz Biotechnology). Arrows indicate the placement of overlaid staining. The results shown are representative of cells from six different experiments (rats). SERT, serotonin transporter. Scale bar=10 μm. (b) Time course for 5-HT uptake in the RA (left) or SMA (right) from pargyline-treated rats incubated with exogenous 5-HT (1 μM) and the effect of fluoxetine (1 μM, 30 min incubation) on 5-HT uptake at 15 min 5-HT-incubation. Bars represent means±s.e.mean for the number of animals in parentheses. *P<0.05 compared with data at time 0 (no incubation). #P<0.05 compared with 15 min 5-HT incubation. RA, rat aorta; SMA, superior mesenteric artery.