Figure 6.

Traces showing typical effects of 10 μM (1-[(2-chlorophenyl)-diphenyl-methyl]-1H-pyrazole (TRAM-34) on endothelium-dependent smooth muscle hyperpolarizations induced by transient exposure to 1 μM calindol (Cal) in endothelium-intact mesenteric artery segments from (a) control (ZL) and (b) diabetic (ZDF) rats. Levcromakalim (LK; 10 μM) was added at the end of the experiment to demonstrate the functional integrity of the myocytes. (c) Graphical representation of four experiments of the type shown in panels a and b. Each column represents the mean (± s.e.mean) membrane potential (m.p.) before and after addition of calindol or levcromakalim in the absence or presence of TRAM-34 as indicated. ^*denotes response in diabetic artery is reduced in comparison to respective control (P<0.05). ZDL, Zucker diabetic fatty rat; ZL, Zucker lean.