Figure 2.
The effects of MG-132 on BMP signalling in colon cancer cells. (a) Shows that BMPRIA and II are expressed in SW1116 and HT-29 cells, as determined by RT–PCR, whereas BMPRIB was only detected in SW1116 cells. (b) MG-132 (1 μmol L−1) time-dependently increased Smad1/5/8 phosphorylation. The expression of total Smad1 was unaffected by MG-132 treatment. The results are representative of two independent experiments. (c) SW1116 and HT-29 cells treated with MG-132 (1 μmol L−1) exhibited stronger fluorescent signalling for phosphorylated Smad1/5/8 than untreated cells (controls), as determined by immunofluorescence staining.