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. 2008 Apr 14;154(3):632–638. doi: 10.1038/bjp.2008.115

Figure 4.

Figure 4

The siRNA-mediated knockdown of BMPRII abolished MG-132-induced BMP signalling. (a) The transfection efficiency of siRNA was determined by transfection of fluorescent-labelled RNA duplex in SW1116 and MKN-45 cells. (b) BMPRII-siRNA downregulated BMPRII mRNA by about 80% when compared with control. (c) siRNA-mediated knockdown of BMPRII abolished the increase in Smad1/5/8 phosphorylation and p21Waf1/Cip1 and p27Kip1 protein expression induced by a 4-hour treatment with MG-132 (1 μmol L−1) in SW1116 cells. The results are representative of two independent experiments. (d) The knockdown of BMPRII reversed the anti-mitogenic effect of an 8-h treatment with MG-132 (1 μmol L−1) on SW1116 cells. *P<0.05; **P<0.005, significantly different from control siRNA-transfected group; †P<0.05, significantly different from control siRNA-transfected group treated with MG-132.