Figure 6.
The downregulation of Smad6 by MG-132 in colon cancer cells. (a) The levels of mRNA for Smad6 were significantly reduced by MG-132 (1 μmol L−1), as determined by real-time PCR. (b) Western blots showed that the expression of Smad6 protein was downregulated by MG-132 (1 μmol L−1). The results are representative of two independent experiments. (c) Knockdown of BMPRII did not abrogate the downregulation of Smad6 mRNA induced by a 4-hour treatment of MG-132 (1 μmol L−1) in SW1116 cells. **P<0.005, significantly different from control siRNA-transfected group; †P<0.005, significantly different from control siRNA-transfected group treated with MG-132.