Figure 2.

Effects of ghrelin (4 μg per rat, i.c.v.), with or without pretreatment (30 min before) with (a) INDO (10 mg kg⁻¹, p.o.), (b) CELE (3.5 mg kg⁻¹, p.o.) or (c) SC560 (5 mg kg⁻¹, p.o.) on gastric mucosal PGE₂ levels determined 60 min after oral administration of 1 mL 50% (vol./vol.) EtOH. The saline group received 1 mL of distilled water instead of 50% EtOH. PGE₂ was extracted from homogenized gastric mucosa and measured by EIA. Each column represents the mean±s.e.mean of at least 10 animals. ^***P<0.001 vs control group; ^○○P< 0.01, ^○○○P<0.001 vs saline+EtOH group; ^§P<0.05 vs ghrelin+EtOH group, ^##P<0.01 vs SC560+EtOH group. CELE, celecoxib; EIA, enzyme immunoassay; EtOH, ethanol; i.c.v., intracerebroventricular; INDO, indomethacin; PGE₂, prostaglandin E₂.