Figure 6.
Effects of ghrelin (4 μg per rat, i.c.v.) on mRNA expression of (a) eNOS and (b) iNOS in gastric mucosa of rats instilled with 1 mL 50% EtOH 60 min before. The saline group received 1 mL of distilled water instead of 50% EtOH. Results are expressed as arbitrary units calculated as the ratio between the OD of either eNOS or iNOS and the OD of glyceraldehyde-3-phosphate dehydrogenase in the same sample. Results are expressed as the mean±s.e.mean of six rats. Representative gel photographs are shown above each column. *P<0.05 vs control group; ○○P<0.01 vs saline+EtOH group. EtOH, ethanol; i.c.v., intracerebroventricular; OD, optical density.