Table 2.
Sizes of the SfiI(fragment)- and I-CeuI(band)-generated restriction fragments from classical V. cholerae 395
| Fragment | Size, kb | Band | Size, kb |
|---|---|---|---|
| A | 364 | 1 | 1378 |
| B | 302 | 2 | 1579 |
| C | 287 | 3 | 330 |
| D | 287 | 4 | 256 |
| E | 259 | 5 | 173 |
| F | 210 | 6 | 119 |
| G | 198 | 7 | 75 |
| H | 189 | 8 | 67 |
| I | 187 | 9 | 6 |
| J | 172 | 10 | 5 |
| K | 150 | ||
| L | 150 | ||
| M | 143 | ||
| N | 138 | ||
| O | 113 | ||
| P | 113 | ||
| Q | 104 | ||
| R | 84 | ||
| S | 75 | ||
| T | 71 | ||
| U | 71 | ||
| V | 65 | ||
| W | 58 | ||
| X | 46 | ||
| Y | 40 | ||
| Z | 31 | ||
| AA | 22 | ||
| BB | 21 | ||
| CC | 12 | ||
| DD | 9 | ||
| EE | 8 | ||
| FF | 5 | ||
| GG | 4 | ||
| Total | 3988 |
The sizes of bands one and two were determined by summation of their SfiI fragments. The mobility of band 2 in the I-CeuI digestion is aberrant relative to the other fragments because of the covalently closed circular nature of this species.