Figure 1.

Autoradiography studies. (a) Autoradiography of brain sections from wild-type (+/+), heterozygote (+/−), and knockout (−/−) mice using p-[¹²⁵I]MPPI, antagonist of the 5-HT1A receptor (Upper) and 8-OH-[³H]DPAT, agonist of the 5-HT1A receptor (Lower), demonstrating the intermediate level and the absence of binding sites in the heterozygote and knockout mice, respectively. (b) Level of p-[¹²⁵I]MPPI binding sites (mean ± SEM, n = four adult male mice) in different structures. Amy, amygdala; Cx, layers IV-VI of cerebral cortex; DR, dorsal raphe, Hip, layer CA1 of the hippocampus; L, lateral septum. (c) 5-HT1A receptor density obtained with p-[¹²⁵I]MPPI and 8-OH-[³H]DPAT in coronal brain section of wild-type (+/+) and heterozygote mice (+/−) expressed as percentage ± SEM of wild-type level (n = four adult male mice).