Figure 2.

Cytotoxic effect of PAO. CHO-K1 (a) and hIRAP-HEK293 (b) cells were treated with different concentrations of PAO for 10 min on ice and 40 min at 37 °C. After being washed, the cells were trypsinized and treated with Trypan blue. Values are expressed as a percentage of the total amount of cells (dead/stained and alive). Data are the average±s.d. of two experiments with triplicate determinations in each. Ang, angiotensin; CHO-K1, Chinese hamster ovary cells; HEK293, human embryo kidney cells; IRAP, insulin-regulated aminopeptidase; PAO, phenyl arsine oxide.