Figure 7.

Translocation of IRAP to the cell surface after treatment of 3T3-L1 adipocytes with insulin. 3T3-L1 adipocytes were incubated with (a) 10⁻⁷ M insulin for different time periods or (b) different concentrations of insulin for 6 min. Subsequently, all cells were further treated as shown in Table 1 (steps 1–3). (c) 3T3-L1 adipocytes were pretreated with wortmannin or LY294.002 and then stimulated with 10⁻⁷ M insulin for 6 min. The experiments were performed as in Figure 5. Data refer to specific binding, expressed as a percentage of the control (no insulin). Values are the average±s.e.mean of three experiments with determinations performed in triplicate. The differences between control and different conditions were determined by using one-way ANOVA and Dunnett's post hoc test: ^**P<0.01; ^*P<0.05; °P>0.05, not significantly different. IRAP, insulin-regulated aminopeptidase.