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Time and sequence effects of DNAzyme. Autoradiogram of an in vitro 1960 nucleotide Egr-1 transcript is shown in both panels, after exposure to active Egr-1 DNAzyme (ED5, upper panel) or its scrambled control (ED5SCR, lower panel). Time-dependent diminution of signal is indicated, with the gradual appearance of the expected 163-nucleotide fragment, only after exposure to ED5. (From Santiago FS et al. Nature Medicine 1999; 5:1264-9, permission pending).
