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. 2008 Apr 21;154(5):1079–1093. doi: 10.1038/bjp.2008.142

Figure 3.

Figure 3

Physical interaction of FP and altFP4 receptors in HEK293/EBNA cells co-expressing FP and altFP receptors. (a) Membrane preparations from control HEK293/EBNA cells (lane 1) and cells stably transfected with HA-altFP4 (lane 2) and cells stably co-transfected with Flag-FP and HA-altFP4 (lane 3) were resolved by 10% sodium dodecyl sulphate-polyacrylamide (SDS-PAGE), transferred onto a polyvinylidene fluoride membrane and probed with anti-HA antibody, HA11. Arrows indicate altFP4 receptor or altFP4 homodimer or FP-altFP4 heterodimer. (b) FP receptors were immunoprecipitated (IP) with anti-HA antibody, from HEK293/EBNA cells transfected with Flag-FP (lane 1), HA-altFP4 (lane 2) or both (lane 3). Proteins were resolved on SDS-PAGE and probed with anti-Flag antibody in immunoblot analyses. Arrows denote FP receptors. HC represents heavy chain. LC represents light chain. HA, haemagglutinin.