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. 2008 Apr 21;154(5):1079–1093. doi: 10.1038/bjp.2008.142

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Copyright 2008, Nature Publishing Group

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/.

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Ca²⁺ signalling of HEK293/EBNA cells co-expressing FP and altFP4 receptors following prostaglandin F_2α (PGF_2α) and bimatoprost treatments. (a) Real-time fluorescence traces were recorded by fluorometric imaging plate reader (FLIPR) in HEK293/EBNA cells co-expressing FP and altFP4 receptors following PGF_2α (10⁻⁷ M) or bimatoprost (10⁻⁷ M) treatment. (I) After recording baseline for 300 s, 10⁻⁷ M PGF_2α was added and recorded for additional 600 s. (II) After recording baseline for 300 s, 10⁻⁷ M bimatoprost was added and recorded for additional 600 s. (b) Real-time fluorescence traces were recorded by FLIPR following PGF_2α (10⁻⁷ M, I) or bimatoprost (10⁻⁷ M, II) in HEK293/EBNA cells expressing wt-FP receptor. Real-time fluorescence traces were recorded immediately following PGF_2α (10⁻⁷ M) or bimatoprost (10⁻⁷ M) treatment. (c) Real-time fluorescence traces were recorded by FLIPR in HEK293/EBNA cells expressing altFP4 receptor following PGF_2α (10⁻⁷ M, I) or bimatoprost (10⁻⁷ M, II) treatment for 600 s. (d) Real-time fluorescence traces were recorded by FLIPR in HEK293/EBNA cells co-expressing FP and altFP4 receptors following PGF_2α (10⁻⁷ M, I) or bimatoprost (10⁻⁷ M, II) treatment in Ca²⁺-free buffer for 600 s. (e) The first peak increase of fluorescence counts was recorded in each treatment (aI–dII). The data represent mean±s.d. of three independent experiments. ^***P<0.01 versus PGF_2α treatment (aI).

Ca²⁺ signalling of HEK293/EBNA cells co-expressing FP and altFP4 receptors following prostaglandin F_2α (PGF_2α) and bimatoprost treatments. (a) Real-time fluorescence traces were recorded by fluorometric imaging plate reader (FLIPR) in HEK293/EBNA cells co-expressing FP and altFP4 receptors following PGF_2α (10⁻⁷ M) or bimatoprost (10⁻⁷ M) treatment. (I) After recording baseline for 300 s, 10⁻⁷ M PGF_2α was added and recorded for additional 600 s. (II) After recording baseline for 300 s, 10⁻⁷ M bimatoprost was added and recorded for additional 600 s. (b) Real-time fluorescence traces were recorded by FLIPR following PGF_2α (10⁻⁷ M, I) or bimatoprost (10⁻⁷ M, II) in HEK293/EBNA cells expressing wt-FP receptor. Real-time fluorescence traces were recorded immediately following PGF_2α (10⁻⁷ M) or bimatoprost (10⁻⁷ M) treatment. (c) Real-time fluorescence traces were recorded by FLIPR in HEK293/EBNA cells expressing altFP4 receptor following PGF_2α (10⁻⁷ M, I) or bimatoprost (10⁻⁷ M, II) treatment for 600 s. (d) Real-time fluorescence traces were recorded by FLIPR in HEK293/EBNA cells co-expressing FP and altFP4 receptors following PGF_2α (10⁻⁷ M, I) or bimatoprost (10⁻⁷ M, II) treatment in Ca²⁺-free buffer for 600 s. (e) The first peak increase of fluorescence counts was recorded in each treatment (aI–dII). The data represent mean±s.d. of three independent experiments. ^***P<0.01 versus PGF_2α treatment (aI).